Characterization of T Cells Immortalized by Taxl
نویسندگان
چکیده
Peripheral blood T cells were immortalized in vitro by introduction of the Taxl gene of human T-cell leukemia virus type 1 (HTLV-1) with a retroviral vector and were characterized for transformation-associated markers. Long-term observation showed that these Taxl-immortalized T cells eventually exhibited very similar features that were characteristic of HTLV-1 -immortalized T cells, ie, increased expression of egr-l, c-fos, IL-ZRa, and Lyn and decreased expression of Lck and cell-surface CD3 antigen. Among these changes, an increase in tho expression of Lyn and a decrease in the expression of Lck and cell-surface CD3 antigen were observed
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